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Compound 3a was also tested and displayed inhibitory potency against AChE-induced Aβ 1 42 aggregation (80.6% and 91.3% at 50 μM and 100 μM screening concentration, respectively).
Compounds 3g and 3n are the most potent analgesic agents from this series, at the screening dose of 100 mg/kg p.o. and compounds 3e, 3j and 3o presented the best antiinflammatory properties at the same screening concentration.
The minimum detectable screening concentration of THC in oral fluid, as measured by ELISA, was 4 ng/ml.
The initial screening concentration of each compound was set by referring to the cytotoxicity assessed primarily with other cancer cell lines.
Seven factors were selected for the screening: concentration of feathers (A), MgSO4·7H2O (B), KH2PO4 (C), CaCl2 (D), yeast extract (E), quantity of inoculum (F) and agitation speed (G), used at two different levels coded as − 1 and + 1 (Table 1).
A primary screen was conducted by the BACTEC 460 radiometric assay at an initial screening concentration of 12.5 µg/ml against M. tb H37Rv (ATCC 27294) in BACTEC 12B medium as reported [30].
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It also investigated the most suitable screening concentrations of FOX, ceftriaxone (CRO) and ceftazidime (CAZ) for the detection of AmpC β-lactamases.
Figure S2 demonstrates that the assay reagents, as formulated at their screening concentrations, were stable over a 24 hr period.
Figure 2 also demonstrates that the assay reagents, as formulated at their screening concentrations, were stable for over 24 hours: both the Z' factor and the signal-to-background ratio remained flat for the duration of the stability test.
Drug screening concentrations (IC25) are determined by generating dose−response curves for each chemical compound.
We determined IC50 values when cytotoxicity resulted more than 50%% at screening concentrations.
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