Sentence examples for screening and cloning from inspiring English sources
In this latter approach, where there is less need to assemble sequences into contigs, the limiting factor becomes the lengths of the fragments that can be obtained for high-throughput screening and cloning.
Herein, we report the screening and cloning of a β-mannanase from actinomycetes isolated in Indonesia to explore its possible utilization as mentioned above.
We thank I. Sures and S. Gärtner for help with cDNA library screening and cloning of mature boophilin, and D. Prasa, H. Pópulo and M.A. Corral-Rodriguez for help with boophilin overexpression and characterisation.
We identified 25 candidate alleles, and cloned and sequenced the duplication/ Ac junctions (the green segment flanking the Ac 5′ end in Figure 1E and Figure 2A) from 16 of the 25 TDD/TDDCI candidates via Ac casting (Singh et al., 2003; Wang and Peterson, 2013) or inverse PCR (iPCR) (See Zhang et al. (2013) for detailed screening and cloning methods).
Wild-type Rab5b (wt) cDNA was amplified by PCR from the pGAD10 construct rescued from yeast two-hybrid screening, and cloned into pCR2.1TOPO.
We have developed a molecular foundation for C. neoformans, including transformation systems, gene disruptions, differential gene expression screens, and cloning pathogenesis genes.
To investigate this we used zebrafish genetic screens and cloned mitochondrial ATPase inhibitory factor 1 (atpifromfrom a zebrafish mutant with profound anaemia, pinotage (pnt (tq209)).
In this study, genes of trans-P4H from diverse sources were screened and cloned into different C. glutamicum strains.
The hybridoma cells were prepared as described above, and antibodies that react with human Notch1-, Notch3-, Norch3- or Notch4-transfected CHO cells, but not with untransfected CHO cells, were screened and cloned.
The 18 432-clone Fragaria BAC library was characterized through the development of pools for PCR-based screening and clones containing markers from the diploid Fragaria reference map were identified.
All clones were blue-white screened and clones were picked randomly for plasmid purification.
