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In these studies, ∼60% of those approached agreed to be screened, and of those identified at high risk after screening, a further 60% agreed to take part in the intervention (a cumulative capture and uptake of 36%).
The possibility of extensive N-terminal truncation of PrPres and consequent loss of the 3F4 and 1E4 epitopes was tested by screening a further replicate Western blot with the monoclonal antibody 94B4 (epitope residues 187 194 of the human PrP sequence).
Following full text screening, a further 67 articles were excluded.
We therefore assume for annual screening a further investigation rate of 24% at first screen and 13% at subsequent.
Following the initial screening, a further 27 candidates were culled because they overlapped higher-scoring candidates, leaving a total of 399 candidates under consideration.
9 However, to tackle the lingering uncertainty that screening immunohistochemistry might be more sensitive than dual enzyme immunoassay screening, a further study to re-test 10 000 of the archived tonsils by immunohistochemistry has been commissioned.
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We therefore screened a further 157 miRNAs for differential expression using the TaqMan® panel.
If we also screened those with a trabecular growth pattern, we would have screened a further 53 cases and found another 5 carriers (10%).
In order to assess the potential role of recessive RYR1 mutations in FADS, and/or MPS we proceeded to screen a further 66 probands for germline RYR1 variants.
In addition, we screened a further 399 and 253 cases, respectively, for specific regions of the FANCD2 and BRIP1/ BACH1 genes that contained functionally important domains, or variants previously found in the germline of breast cancer cases.
We also used this approach to screen a further 11 E-boxes lying in the Pdx-1 promoter region between −2.7 and −0.26 kb (Fig. 3) but could not reveal evidence for ChREBP (not shown).
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