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A total of 148 bait clones (9.6% of all screened baits) were screened in the presence of 50 mM 3-AT (Supplementary Table 4).
The ability of bacterial strains to produce detectable amounts of CMCase under catabolic repression (genetically) was screened in the presence of glucose (1 % w/v) equivalent to CMC as sole carbon source.
Thirteen agents previously described as promoting C2C12 differentiation in the absence of TGF-β1 were screened in the presence of TGF-β1.
Selected hits are then used in a second screening campaign in which a second library of fragments (a few hundred) is screened in the presence of a saturating concentration of the first hit.
We screened in the presence of 200 nM ABT-737 as this concentration was >10-fold less than the IC50 in parental cells and ∼10-fold greater than the IC50 following Mcl1 knockdown, demonstrating maximally divergent cytotoxicity.
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One may choose to optimize any crystallization hit (obtained by screening in the presence of heterogeneous nucleating agents) either with or without the nucleant.
Alternatively, a crystallization hit obtained by screening in the presence of heterogeneous nucleating agents provides material for traditional seeding approaches using homo-nucleation [24].
One hundred and sixty-eight apparently wild-type samples from male patients were then re-screened in the presence of normal DNA, and this revealed an additional 10 variants.
Finally, screening in the presence of other BET proteins can increase the stringency and binding information for finding selective BET inhibitors.
Alonzo and Pepe recently described another method for computing the AUC of a continuous screening test in the presence of verification bias.
Patients were allocated to treatment by the author opening the next numbered envelope, after screening, in the presence of the patient.
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