To isolate novel hypophysiotropic neuropeptides, Miyata, Arimura and colleagues screened fractions from an extract of ovine hypothalamic tissue (4370 pieces; 2400 g) by monitoring their AC activity in cultured rat anterior pituitary cells.
Of the crude extract and fractions screened, ethyl acetate fraction of E. alba inhibited adipocyte differentiation in 3T3-L1 pre-adipocytes and hMSC derived adipocytes.
Among men who were screened, a fraction of 1.05% (=2.94 1.89%) did not die of prostate cancer because the cancer was detected and treated earlier (Table 2).
Based on the better result, the ethyl acetate extract was selected and purified through silica column chromatography and screened the fractions for antimicrobial and antitumor activities.
DOI: http://dx.doi.org/10.7554/eLife.00683.004 To identify SRBF1, we purified it by column chromatography, and screened individual fractions by gel mobility-shift and RNA cross-linking assays.
We screened for fractions containing the full complement of 14 subunits by running the treated samples in a 10% SDS-PAGE gel and silver-staining it.
Main outcomes were: (1) eligibility fraction (proportion eligible of those screened), (2) enrolment fraction (proportion randomised of those eligible), (3) recruitment fraction (proportion of potential participants actually randomised), and (4) number of patients needed to be screened (NNS) in order to randomize one participant.
Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol- 2-4 ]-diphenyltetrazolium 5-dimethylthiazol- 2-4 ]-diphenyltetrazolium
Among the candidates that were visually screened, a large fraction (62%) expressed the R11C05-LexA tester incompletely, as they lacked expression in one or more of the scored brain regions.
By screening the fractions in this manner it was possible to determine that only two of them (fractions 25 26 and 26 27 minutes, respectively) contained glycopeptides.
