Using this yeast assay, we screened expression libraries for cDNAs from the plant, Lycopersicon esculentum (tomato), and the invertebrate animal Drosophila melanogaster (fruit fly), identifying close homologs of BI-1 as Bax-suppressors.
To this end, we first screened expression of ERBB receptors in mouse MSCs and determined that NRG1 and ERBB2, but not ERBB4, were expressed in MSCs.
Next, we screened expression of SRC1, TIF2, PIAS1, PIASx, ARIP4, BRCA1, β-catenin, AIB3, AIB1, CBP, STAT1, NCoR1, AES, cyclin D1, p300, ARA24, LSD1 and BAG1L coregulators in triplicates (three independent Q-RT-PCRs' runs).
To test if miRNA pathways crosstalk with the pharmacologic action of bufalin in cancers, we screened expression of some cancer-related miRNAs in PC-3 cells after bufalin treatment, and observed miR-181a expression to significantly increase in a dose-dependent manner.
In our previous study, we screened expression of different miRNAs in archived formalin-fixed and paraffin-embedded breast tissue specimens by in situ hybridization [ 10, 28], we found significant downregulated miR-133a expression in breast cancer compared to benign breast disease and miR-133a downregulation was associated with disease progression.
Therefore, the final number of tested hypotheses is equal to the number of screened expression data sets.
It was therefore the aim of the study to screen expression of metabolic proteins mainly represented by metabolic enzymes in the hippocampus over the estrous cycle and in males.
ZAS3 has been cloned by screening expression cDNA libraries using the V(D J recombination signal sequences or the calcium-binding protein S100A4/mts-1 genhancerncer [3], [4].
Total RNA (1 µg) isolated (Trizol Reagent, Invitrogen) from fresh arcuate nucleus tissue was converted to cDNA and used to screen expression levels of Sirt1, POMC, AgRP, and tubulin (internal control).
Consequently, whether pollen allergens are identified by immunoblotting soluble protein extracts from commercially washed pollen [31], [32] or by screening expression libraries derived from pollen cDNAs with patient sera [33], pollen surface materials are likely to be overlooked.
EmBP1, expressing in the mature wheat embryo and binding to Em1a motif, was cloned by screening expression cDNA library using [P]-labeled Em1a as probe.
