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Therefore these five traits could be used as a combination to screen wheat germplasm or lines for drought tolerance in wheat breeding program.
To gain insight into PSY gene expression during grain development, we used Single-Strand Conformation Polymorphism (SSCP) analysis [ 27] to screen wheat RNAs (cv Recital) extracted from leaves, roots and grains at different development stages.
The aim of this study has been to use Fourier transform infrared (FTIR) spectroscopy and Partial least squares (PLS) modelling to rapidly screen wheat cultivars for the levels of component tissues, the carbohydrate composition and lignin content, and the levels of simple cross-linking phenolics such as ferulic and diferulic acids.
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Upon initial screening, wheat bran and ammonium chloride were chosen as suitable carbon source and nitrogen source respectively.
In our hands, the method was successful where cDNA library screening with heterologous probes (e.g. cDNAs from maize for screening wheat libraries) had failed.
To screen the wheat specific miRNAs, we explored the presence of 323 novel wheat miRNAs across Arabidopsis, soybean, rice, maize, sorghum, barley and Brachypodium.
We show here that TILLING constitutes an effective way to screen large wheat mutant libraries for induced polymorphisms.
The use of hyperspectral imaging (HSI) for deoxynivalenol (DON) screening in wheat kernels is investigated.
Kits designed to detect ochratoxin A (OA) and T-2 toxin by a membrane-based flow-through enzyme immunoassay were studied collaboratively by screening cereals (wheat, rye, maize and barley) for the presence of these mycotoxins.
We thank Dr Chunji Liu for providing seed for screening multiple wheat genotypes.
In total, 650 stable transformants were generated and screened on wheat ears of the cultivar Bobwhite for reduced virulence using previously described methods [ 15].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com