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Interestingly, our more successful CRM screen utilized the opposite approach, looking for dissimilar neighboring sequences.
Both phases of the screen utilized the Smn73Ao allele, which gives a robust NMJ defect, and importantly, contains a point mutation in the YG box (Figure 2A), which is the location of a documented human SMN1 mutation [3].
We are aware that the set-up of the primary screen utilized here does not differentiate between compounds that act through the caspase-8 axis and those that do so and also sensitize with TRAIL.
The screen utilized fluorescent lipid analogs to directly assay intestinal lipid absorption in larvae treated with novel chemical compounds, thus distinguishing it from a study that examined the effects of known drugs on endogenous yolk-lipid metabolism in younger zebrafish larvae [39].
The screen utilized cultured fascin-deficient mutant Drosophila neurons, whose neurite arbors manifest the 'filagree' phenotype.
The first screen utilized BLASTP to identify orthologs in the respective proteomes.
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Agents were, thereafter, quantitatively assessed for their ability to suppress the synthesis on TNF-α in a lipopolysaccharide (LPS -challenged mouse macrophage-LPS -challengedcreen, utilizing cultured RAW 264.7 cells.
To identify previously unrecognized deleted genes or loci that can lead to MSI, we developed a functional genomics screen utilizing a plasmid containing a microsatellite sequence that is a host spot for MSI mutations and the comprehensive homozygous diploid deletion mutant resource for Saccharomyces cerevisiae.
To investigate the function of XB21, we performed a yeast two-hybrid screen utilizing XB21 as bait with a rice cDNA library.
We conducted a reverse genetic screen utilizing morpholino oligonucleotides previously designed and validated against ten known zebrafish Notch pathway members [15] [19].
Based on the results, a screen utilizing the assay developed here would be optimally conducted at drug concentrations up to 1µM.
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