Sentence examples for screen substrates from inspiring English sources
Finally, using high-content screening methods incorporating GFP-fused cargoes, it might be possible to screen substrates of CDK1 115 for those that affect the protein transport process.
By coupling the technique of SECM with a light source, we have examined the surface charge of non-earthed pseudo-DSCs under variable light conditions and screened substrates by varying the working electrode potential.
The screened substrates were then stored in airtight plastic bags at room temperature until use.
Promising mutants were probed using both diastereoisomeric screening substrates (4 S)- and (4 R)- 4 in order that genuinely selective enzymes were identified.
About 1% of library members showed significant differences in activity between the two diastereoisomeric screening substrates, (4 S)- and (4 R)- 4. Two variants were selected as displaying greater stereoselectivity than the E192N 'parental' variant.
In a screen for substrates of these kinases involved in Snail regulation, we identified the scaffolding protein DIXDC1.
We combined mass spectrometry and affinity column chromatography of the catalytic domain of protein kinases to screen potential substrates.
To screen potential substrates of Rho-kinase, we examined whether the active catalytic fragment of Rho-kinase (Rho-kinase-cat) interacts with its substrates by affinity column chromatography.
Although the above-described approach provides a fast, sensitive means to screen for substrates of potential acyltransferases, individual lipid species were further analyzed after normal-phase LC separation.
Consistent with this idea, MVB12A was recently identified in a proteomics screen for substrates that are tyrosine-phosphorylated in response to epidermal growth factor (EGF) stimulation, and was shown to function in EGF receptor downregulation [27].
Thus this shRNAs mixture can be regarded as a specific inhibitor of CYP3A4, which can be utilized to screen the substrates or introducer of CYP3A4.
