A synergy between these advances and the development of screening tools for a rapid and reliable screening of marker gene expression represents an important step towards an improved treatment of cancer.
Finally, while chloroplast DNA presents limited variability in Ficus [ 72], our initial screening of markers allowed increasing the number of polymorphic sites.
First, NGS allows for genome-wide screening of markers, which may lead to a more representative estimation of genetic variation within and between populations.
During the process of screening of markers for population genetics, four of seven PCR-RFLP markers previously used for genotyping isolates of R. solani AG-3 from potato (pP42, pP46, pP47 and pP89) also amplified genomic DNA from R. solani AG-3 from tobacco.
Our aim was to develop and test microsatellite markers by combining the 454 sequencing with the screening of markers using the QIAxcel gel electrophoresis system (QIAGEN, Melbourne, Victoria, Australia; Gross et al., 2012) before labeling with the cost-effective M13 universal labeling technique.
Another recent study carried out a genome-wide screen of microsatellite markers in 360 patients with a variety of cancers and identified a marker in the promoter region of SEM3A (required for neuronal development) associated with acute radiotherapy toxicity [ 62].
In a screen of candidate markers for the detection of prostate carcinoma cells in coculture with endothelial cells, we found that CDw75 was a specific marker for all three prostate cell lines under study.
In addition, we further evaluated molecular mechanisms of action of pralatrexate, and screened for markers of sensitivity and acquired resistance to pralatrexate, knowledge of which could be of potential use for designing future clinical trials.
Additionally, in a population genomic screen of thousands of markers in wild steelhead and rainbow trout from Sashin Creek, Hale et al. (2013) also identified loci on Omy12 and Omy14 that contribute to the genetic differentiation between migrants and residents.
A screen for markers of PTEN identified IGFBP-2 as being the most significantly associated marker (Mehrian-Shai et al, 2007).
