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To isolate full-length cDNAs encoding the genes of interest, the initial partial clones obtained from the differential screen library [35], [36] were used to screen a stage 2 4 chick cDNA library (kind gift of J.C. Izpisua-Belmonte), directionally cloned in Uni-Zap (Stratagene) under standard stringent conditions (0.1 X SSC, 65°C).
Screen library for non-functional, weak, moderate, and strong promoters.
The chemical, toxicological and clinical data of this screen library were compiled.
Notably, many of these processes can be studied using cultured cell assays, making the gene set particularly relevant for development of a high-quality cell-based RNAi screen library.
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The choice of screen libraries such as scale, type, or format can be determined based on biological questions or the phenotypic assays developed.
Likewise, MLSMR is a non-redundant screening library by design.
Successes of such screening campaigns critically depend on the quality of the screened library.
This is probably due to particular reaction types used to construct the screening library.
Many factors should be considered while selecting an optimal screening library.
Bonferroni correction was used to correct p-values with respect to the size of the screening library [5].
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