All dsRNA lines used for the modifier screen were obtained from VDRC and the Harvard Drosophila stock collection (Supplementary Table 2).
At the screening visit, subjects provided a detailed medical and headache/migraine history, a physical examination was performed, laboratory assessment samples (ie, hematology, serum chemistry, urine analysis, serology, urine drug screen) were obtained, inclusion/exclusion criteria were verified, and a 12-lead electrocardiogram (ECG) was completed.
The gray level-luminance function of the screen was obtained with a psychophysical method of gamma correction (Bordier C. & Knoblauch K., personal communication).
A scatter plot for the primary screen was obtained by plotting the 2D normalized luciferase ratios for a clone in the first experiment against that obtained in the second experiment (Figure 2A).
All studies deemed potentially relevant after the screen were obtained, and the full manuscripts were reviewed for further exclusion.
First, the miRNA count data in this preliminary screen were obtained prior to the finding that there can be significant RNA ligase-dependent bias in small RNA cloning.
The deletion strains for the screen were obtained from the Bioneer version 2.0 library, which includes 3005 mutants (∼84% of all non-essential genes).
Commercially available replicates and derivatives of molecules identified as hits in our HTS screen were obtained from Ambinter (Orleans, France) as dry powders.
Study Design: DEXA measurement (with the result expressed as T-score) was performed in 79 women in whom Pap smears for routine cervical cancer screening were obtained.
Brain magnetic resonance imaging and routine blood work-up with erythrocyte sedimentation rate and immunological screening were obtained with no abnormalities.
