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In this study, we took advantage of the unique endo-lysosome system in macrophage-like cells in Caenorhabditis elegans (C. elegans) to screen for natural compounds that induced lysosomal abnormality.
Plug and overlay assays were applied to screen for natural isolates that modify selection of tetracycline resistance using a Type II assay (∼700 by plug assay; ∼60 plates of about 50 colonies each using the overlay assay).
To further pilot our approach, a screen for natural selection imprints on the genomic neighborhood of 18 genes previously reported as objects of historic section validated IL4 (Figure 2C), plus seven more (IL13, ALDH2, SIGLEL1, SIGLEL9, FOXP2, CCR5, and AGT) as demonstrating "recent" or "old" selection patterns (Table S1, Notes S1, and Supplemental References S1).
To screen for natural compounds that up-regulate nuclear transcription factor Foxp3 expression in T cells, HEK 293 T cells were transiently transfected with the Foxp3-GFP expression plasmid pZIGF.
To create bona fide facultative cooperation, we would need to either screen for natural microbes for facultative cooperation or create reduction-of-function (rather than loss-of-function) alleles in enzymes mediating adenine and lysine biosynthesis, and ideally these mutations should also be non-revertible.
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These primers will be useful in screening for natural variation in the triticin HVR segment in wild wheat collections.
The genetic approaches taken include selection (for heat tolerance and resistance to nematicides); screening for natural isolates displaying heat tolerance; hybridization (aided by genetic markers) and mutagenesis for generating mutants displaying either desired beneficial traits or marker mutations.
Molecular docking was first performed as a primary screening for natural compounds targeting Annexin A2.
A total of 92 tomato lines of the EU-SOL core collection were screened for natural variation in exons 1 5 and introns 2 and 4, based on PCR amplification of three amplicons of the SleIF4E gene (Figure S3) from 20 (12X and 8Y) 2D pools using tagged PCR primers.
PubChem database was screened for natural compounds possessing antineurodegenerative potential.
In fish breeding, it is essential to discover and generate fish exhibiting an effective phenotype for the aquaculture industry, but screening for natural mutants by only depending on natural spontaneous mutations is limited.
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