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A screen for evolutionary conservation with SIFT v.2.0 predicted this substitution to be tolerated [ 32].
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Any genome wide screen for large-scale evolutionary change will enrich for false positives.
In this study, to explore the correlation between hydrogen peroxide (H2O2) resistance and growth benefits in mycobacteria under stress, we developed a model for evolutionary screening of H2O2-resistant mutations in a pathogenic mycobacteria model, M. smegmatis, using a clinically important stressor, H2O2 (Fig. 1).
Thus, our evolutionary screen for novel restriction factors among the TRIM gene family identified 15 members not previously known to be under positive selection.
These are screened in the next step for evolutionary conserved and thermodynamically stable structured ncRNAs using RNAz [ 11].
Stabilization is not the only objective for which the approach is useful, and others could include the selection of residues for nondisruptive destabilizing mutations to reduce the lifetime of proteins, or to generate proteins that can be used to screen for second-site revertants in evolutionary engineering strategies.
To our knowledge, this is the first broad unbiased (i.e. not driven by evolutionary conservation) screen for transcriptional enhancers in transgenic mice.
For the purposes of drug design, the temptation to screen for potential target genes by applying evolutionary analyses to whole coding sequences represents a serious potential pitfall.
Despite their evolutionary relationship, our screen for positive selection in primates highlighted TRIM52 but not TRIM41.
An increasingly popular approach to study the role of selection for camouflage for evolutionary trajectories of animal colour patterns is to present images of prey on paper or computer screens to human 'predators'predators
That is good news for evolutionary biologists.
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