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Yesterday, China said that it had begun carrying out nationwide inspections of wheat gluten to screen for contamination.
No-template controls were included in each experiment to screen for contamination.
No template controls were included for each real-time PCR run to screen for contamination of reagents.
To screen for contamination, we BLASTed nucleotide unigenes against NCBI's non-redundant protein database (nr) (e-value < 0.0001), producing a lineage assignment for each EST.
In order to screen for contamination of reagents or false amplification, PCR controls were prepared for each sample by preparing identical reaction mixtures except for the addition of the no template control (NTC).
In order to screen for contamination of reagents or false amplification, PCR controls were prepared for each sample by preparing identical reaction mixtures except for the addition of the template (NTC).
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After screening for contamination, three scaffolds were identified as putative contaminating sequence of possible bacterial origin and removed (Additional file 1: Table S1).
The images of families being screened for contamination by workers in protective suits add to the sense of alarm.
The samples were screened for contamination using high soluble reactive P (>0.2 mg) as an indicator (Lohse et al. 2008).
For organ outgrowth assays, tissues from wild type (WT), collagen X Tg and KO mice were aseptically harvested, cut in half for duplicates, placed in 12 well tissue culture plates (TKR Biotech products) with 1 ml sterile RPMI media with 10% FBS (Gibco), incubated at 37°C for 3 6 days, and visually screened for contamination every 12 hr.
To ensure removal of the 454 key sequence from reads, 10bp were trimmed from the 5′ ends and screened for contamination using the vector/adapter file.
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screening for contamination
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screen for club
screen for cage
screen for depression
screen for loneliness
screen for work
screen for performance
screen for program
screen for example
screen for registration
screen for normality
screen for pneumonia
screen for comment
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