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A previous study in yeast had also identified a tah18 mutant through a genetic screen for conditional tah top1T722A-hypersensitiveexhibiting exhibithermo-sensitiveitive growth at 36°C in the presence of the camptothecin mimetic mutant top1T722A [25], [26].
Besides G127D, only one other yeast rpb2 region A mutation has been reported, R120C, which was isolated in the Young laboratory in a screen for conditional mutants (Scafe et al. 1990a).
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Since the initial establishment of this nematode as a model organism, screening for conditional C. elegans mutants has been more feasible than in other animals, in part because it is self-fertile [21], [22], [23].
Clones were screened for conditional expression of βglu and βgal, respectively, in the presence of Pronesterone A (5 μM).
Targeting of these pre-assembly Nups to the NE seems to require certain soluble transport factors normally used to chaperone and power the transport of cargoes through the NPC (see below), as genetic screens for conditional mutants in NPC assembly identified Ran, RanGEF, RanGAP, and Ntf2 (see Figure 4 and below) (Ryan and Wente 2002; Ryan et al. 2003, 2007).
The ability to screen for survivors starting from a conditional lethal strain is rapid and convenient.
Finally, multiple liver tumours, morphologically similar to those generated using a conditional SB11 screen for hepatocellular carcinoma [ 27], were observed in 19 mutagenised Ptch+/- mice but not in predisposition controls.
Mutants for conditional epistatic miniarray profiling (cE-MAP) screens were constructed in the BY4742 background, whereas all other strains were constructed in the W303-1A background.
This approach allows for conditional selection and can be used for screening human cDNA libraries to identify factors that are required for Sec incorporation.
Because the association was supported in subsequent meta-analysis incorporating this and four more GWAS datasets, 10 we undertook fine mapping and conditional analyses to screen for risk variants with stronger and/or independent signals of disease association.
He has no use for conditional supporters.
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