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Small molecule screen for cold senstivity.
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In this study, recombinant inbred lines (RILs) derived from a cross between a cold sensitive variety, Dongnong422, and strongly cold-tolerant variety, Kongyu131, were used to screen for cold-tolerant loci at the booting stage of rice.
Screening for cold responsive genes in P. euphratica can be a useful approach to understand the responses of woody plants to low temperatures and can also help elucidate the difference in cold perception between below- and above- freezing temperatures.
Therefore, compounds 2 5 were screened for cold-flow performance through the determination of their cloud and pour points.
Alleles of CDC11 had also been found in similar screens for cold-sensitive (Cs–) mutants (Moir et al. 1982; Healy et al. 1991), so we analyzed four of these.
Importantly, this screen was also successful in identifying the vast majority of previously reported cold sensitive ribosome biogenesis genes (Supplementary file 1B), providing support that screening compounds for cold sensitivity would enrich for those related to ribosome function and biogenesis.
Plants for cold tolerance screening were irrigated with 24°C water until the tillering stage (30 days after transplanting) and then treated with 17°C cold-water until maturity.
Why are commercials for Coors Light beer, sold by MillerCoors, centered on the concept that new Cold Activated Cans provide "cold insurance" when all the spots include disclaimers superimposed on screen that declare, "There is no insurance for cold beer"?
(A ) Screen of the E. coli Keio collection for cold sensitivity.
We screened for drought and cold response independently and selected the ESKIMO1 mutant in both screens.
(A ) Screen of ∼30,000 small molecules at 10 μM against E. coli for cold sensitivity.
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