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To this end, we developed CrossCheck, an online platform for high-throughput screen data analysis.
E.M. and A.B. helped with the CRISPR-Cas9 screen design, library preparations, sequencing, and supervised the screen data analysis.
Our efforts in bioinformatics grew from the need for state-of-the-art reagent design and screen data analysis approaches.
CrossCheck dramatically simplifies high-throughput screen data analysis and enables researchers to dig deep into the published literature and streamline data-driven hypothesis generation.
For the CRISPR screen data analysis, the statistics were calculated from a comparison of read counts with the respective representation sample using the software package MAGeCK73 version 0.5.3.
Many factors influence screen outcomes, including cell line selection, screen format, type of reagent, coverage of genes by the reagent library, cell assay, and screen data analysis.
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Xi Kathy Zhou, Ph.D., M.S., joined the faculty at Weill Cornell Medical College from the Genomic Institute of Novartis Research Foundation in San Diego, where she served as a biostatistician and worked on projects related to microarray and high-throughput screening data analysis.
Initial lecture workshops cover the basic principles; then students, in teams, seek to improve the profile of a weakly potent, insoluble phosphatidylinositide 3-kinase delta (PI3Kδ) inhibitor (1) through compound array design, molecular modelling, screening data analysis and the synthesis of target compounds in the laboratory.
KMW performed newborn screening data analysis and helped to draft the manuscript with respect to biochemical issues.
AG participated in the design of the study, performed newborn screening data analysis and helped to draft the manuscript.
Statistical methods used for HT screens data analysis are commonly borrowed from small-molecule screens or even other types of high-dimensional data methods.
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