It also investigated the most suitable screening concentrations of FOX, ceftriaxone (CRO) and ceftazidime (CAZ) for the detection of AmpC β-lactamases.
Figure S2 demonstrates that the assay reagents, as formulated at their screening concentrations, were stable over a 24 hr period.
Figure 2 also demonstrates that the assay reagents, as formulated at their screening concentrations, were stable for over 24 hours: both the Z' factor and the signal-to-background ratio remained flat for the duration of the stability test.
At the same time, the coral response to sunscreen exposure was not dose dependent, as the same effects were observed at low and high sun-screen concentrations.
Drug screening concentrations (IC25) are determined by generating dose−response curves for each chemical compound.
We determined IC50 values when cytotoxicity resulted more than 50%% at screening concentrations.
The local concentration or density of compounds on the surface of TentaGel beads is very high compared to standard screening concentrations in homogenous solutions.
For the cancer dataset, we predict AUC within the range of experimental screening concentrations for each cell line against 138 anticancer drugs using gene expression profiles.
We choose to perform our analysis on AUC values because IC50 values are not observed before the maximum screening concentration for a significant proportion of the drug and cell line pairs (i.e. most of the cell lines are resistant to a given drug within the range of experimental screening concentrations).
The compounds that induced developmental toxicity effects and caused embryo death in the primary screening were re-tested in cldnb EGFP embryos by using final screening concentrations of 0.5, 1 and 5 μM.
This procedure yielded a total volume of 120 μl per well and resulted in the respective final compound screening concentrations for this assay (10 μM ibuprofen, 3 μM diclofenac, 10 μM indomethacin, 1 mM dexamethazone, 300 μM hydrocortisone).
