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The migration of cells was analyzed using scratch wound migration assay.
Scratch wound assays also showed reduced migration in RD cells expressing exogenous TBX3 (Fig. 4d).
Cell mobility was assessed using a scratch wound assay.
We used two different paradigms for the scratch wound assay.
Initially, a monolayer scratch wound assay was performed.
A scratch wound was made the next day using a plastic pipette tip.
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Scratch wound-healing was performed essentially as described.
The same results were visually confirmed with scratch wound-healing assay.
To investigate the effect of NO on cell migration, we performed scratch wound-healing assays.
Thus, scratch wound-healing assay was performed to evaluate cell migration in FTC133 cells.
Cell migration was also analysed in scratch-wound assays on confluent monolayers of DU145 cells.
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