Briefly, tumor cell-rich areas in 0.1% methylene blue-stained 10-μm sections under microscopy were scratched with 20 gauge needles and deparaffinized.
To examine this theory, the HI-SEAS crew members on Mauna Loa are testing their sense of smell two different ways: they take scratch-and-sniff tests, which gauge the performance of their sense of smell, and odorant-I.D. tests, in which they try to identify a piece of food that is placed in a small, opaque tub by sniffing through a straw.
Just as it is important to strut your scratching stuff, it's integral to gauge the audience's reception to your soundtrack.
It was then scratched with a 30-gauge syringe needle in PBS containing 1.5 mM Ca2+ or no added Ca2+.
Cultured monolayers were scratched with a 30-gauge syringe needle in the presence of FDx (10 mg/ml) dissolved in PBS (containing 1.5 mM Ca2+), the culture was then washed 3 times with PBS, and FM 4-64 (2.5 µg/ml, in PBS) added.
Two-tailed significance was established at a confidence level of 0.05 ≤ P ≤ 0.95. 1. Experimental a. Installation of 20 ul containing 4 × 107 pfu Ad-5 into the anterior cul-de-sac and scarifying the conjunctiva (4 scratches) with a 25-gauge needle (Group "C+": 8 eyes).
Scratch assays were then performed in HUVEC monolayers to gauge the effect of the various MV conditioned medias on endothelial cell migration.
For dermal scarification, 1×106 PFU VACV-WR or vGK5 in 50 µl PBS was placed at the base of the tail and 10 20 scratches were made in a crosshatch pattern using a 21 gauge needle.
In order to gauge how long TG2 protein remains active after wounding, scratches were made 48, 24, 12, and 0 hours before adding the TG2 substrate 5-BP.
Leaves were lightly wounded on the adaxial leaf surface with a 2 3 mm shallow scratch (without complete puncture) on either side of the mid-vein with a 27-gauge syringe needle [21], [22], [23].
The newness of the industry has academics scratching their heads, saying that they can only look toward the history of timeshares to gauge how these homes might do on the resale market.
