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Scratch assay was performed as previously described54, 57.
(a) Cell migration was detected using wound scratch assay.
(a) Representative images of wound healing scratch assay in A549 cells.
The scratch assay was performed on 4 replicate cultures for each condition.
Further, we observed an increase in proliferation using a scratch assay in siHopx-transfected MLE12 cells (Fig. 3F,G).
Fig. 3 Cell migration analyzed using scratch assay.
Corneal endothelial cells migration was evaluated by scratch assay and analyzed with Axiovision software.
In the scratch assay, PRFexu and PRFext were the most effective for wound closure.
The supernatant was further filter-sterilised before use in a wound scratch assay.
The scratch assay was performed with MiaPaCa-2 cells, overexpressing various constructs.
However, abundant TG2 activity was detected around the wound in a standard cultured fibroblast scratch assay.
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