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Autophagy is the body's internal recycling programme - scrap cell components are captured and the useful parts are stripped out to generate energy or build new cells.
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A liquid nitrogen cooled spatula was used to scrap cells from the membrane followed by immediate flash freezing in an RNase-free 50 ml conical tube containing liquid nitrogen.
Gently scrapped cells were resuspended in PBS and analysed by flow cytometry.
Next day, a 50 μL aliquot of scrapped cells was incubated in 40 mL of LB (100 μg/mL Ap, 1% glucose) till OD600 = 0.2 with shaking at 37°C.
Cells are removed from the dish/flask by scrapping the cells.
Virtually all the metal can be recovered from scrapped mercury cells, mercury boilers, electrical apparatuses, and control instruments.
After scrapping, the cells were centrifuged and the supernatant was employed for ALA and PBG determinations.
After scrapping, the cells were centrifuged and the content of two dishes was pooled.
After scrapping, the cells were centrifuged and the supernatant was employed for ALA and PBG determination following the method of Mauzerall and Granick (1956), slightly modified as indicated.
After scrapping, the cells were washed with PBS twice, and then incubated with fresh medium containing VEGF with or without ellagic acid (10 μM).
He doesn't even want to talk about his crime, unlike the killer in his neighboring cell, Scrap (Shane McRae), whose guilt hangs upon him much more conspicuously.
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