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A scrambled sequence (AllStars negative control siRNA; Qiagen) served as control.
The sequences for hsa-miR-520d-5p hsa-miR-520d-5p hsa-miR-520d-5p hsa-miR-520d-5pandCCUUUC-3′ and 5′-GAGUCCGCCtheATAGACAA-3′, rescrambledy.
PC3 cells were transfected with siRNA against a scrambled sequence (Ctrl) or HSF2, and cultured in 3D.
For control purposes, a scrambled sequence of 26RFa (Sc-26RFa) and normal saline (1 ml) were injected.
Expression of AT1R/EYFP with ECFP/AIIC (control scrambled sequence AII fused to ECFP) has no effect upon cell proliferation.
(a) Western blot of the silencing efficacy in PC3 cells 48 h after transfection with siRNAs against a scrambled sequence (Ctrl), HSF1 or HSF2.
(b) SkBr3 cells stably expressing shRNA against a scrambled sequence or PIPKIα were fixed and immunostained with PIPKIα and p53 antibody.
The use of a scrambled sequence of the VRPMPLQ peptide and competition studies, employing excess of the free peptide verified the specific nature of the NP cellular uptake.
The potential of the dendrimers as nanocarriers for anticancer Bcl-xl, Bcl-2, Mcl-1 siRNAs and additionally a scrambled sequence siRNA has been explored.
SiRNA with scrambled sequence was used as the control.
Non-targeting siRNA (NT-siRNA) was synthesized by a scrambled sequence of nucleotides as a control siRNA.
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