Sentence examples for scramble insert from inspiring English sources
Notably, empty vectors may lack the control over the non-specific effect of the precursor transcripts but they were able to reduce the off-target effects of a scramble insert.
Briefly, the predicted miR-467b-5p miR-467b-5p miR-467b-5p74-380 of Slc17a6 3′UtargetBI:NM_080853) or a mutated target site (Scramble) was inserted into the 3′UTR of a firefly luciferase repositionene.
The authors acknowledged that gene gun insertion can cause "deletion and extensive scrambling of inserted and chromosomal DNA".
A scramble sequence was inserted into pGPU6 RNAi plasmid and used as control shRNA (NC).
As a control we inserted a scramble shRNA sequence into the same vector (GAA CAA TCT GTG CTT ACC CAT).
The same vectors with inserted scrambled shRNA expression cassettes served as negative controls.
Additional necessary control for the Luciferase experiments is a pGL- SV40) pGL- SV40vector harboring an insert of a scrambled sequence that its size range mimics the average insert size of the tested ECRs.
By this year, the Sigint Enabling Project had found ways inside some of the encryption chips that scramble information for businesses and governments, either by working with chipmakers to insert back doors or by exploiting security flaws, according to the documents.
Results shown are the mean of three experiments and expressed as a fold increase over un-induced scramble controls (migration) or as invasion index (compared to and corrected for control inserts).
Figure 4d shows that firefly luciferase activity was significantly decreased when the putative target site of miR-467b-5p miR-467b-5p miR-467b-5po the reporter compared to vector only or the Scramble construct.
For positive selections we used the DDX3 insert peptide (GSGSGS-HHHHHH-GSGSGS-ALRAMKENGR-GSGSGS-EQKLISEEDL), for negative selections a scrambled version of the DDX3 insert (GSGSGS-HHHHHH-GSGSGS-RKAEGLNARM-GSGSGS-EQKLISEEDL), each with a His-tag for immobilization and a Myc-tag for detection (tags are in italics, the actual DDX3 peptide sequences in bold).
