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(Scramble 1 and 2 were used for immunization, Scramble 3 was utilized during purification to decrease the isolation of irrelevant antibodies to these linker sequences).
Additionally, it was confirmed by ELISA that the purified antibodies showed little or no binding to the sequences found in Scramble 1 or 2, or OMPC.
Thus the antibodies purified would be those specific to the caspase substrate tetrapeptides, while the antibodies recognizing linkers Scramble 1 or 2, or the carrier protein OMPC, would not bind to the resin.
Three different octapeptide linker sequences (Scramble 1, 2 and 3) were chosen to closely resemble native rabbit peptides in order to minimize generation of antibodies to these irrelevant parts of the peptide.
Each of the eight peptides was coupled to two different linker sequences (designated Scramble 1 and Scramble 2), chosen to closely resemble native rabbit peptides in order to minimize generation of antibodies to these irrelevant parts of the immunogen.
The cohort of rabbits immunized with the mixture of eight Scramble 1 peptides were boosted once with the Scramble 2 peptides, and vise versa, such that the only commonality between immunization and boost was the c-terminal DXXD sequences.
Similar(53)
According to (12), if we choose Φ i to scramble y1 and y2 directly, the two measurement parts cannot be separated for decryption since Null Φ1) does not exist.
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The retroviral vectors (scramble, shp300, and shp53) used in this study were purchased from Origene Technologies, U.S.A.
Eight Scramble 1-DXXD (1.25 mg each) or the eight Scramble 2-DXXD conjugates (1.25 mg each) were combined to create antigen set A (the pool of all eight OMPC-Scramble 1-DXXD conjugates) and antigen set B (combination of 1.25 mg of each of the OMPC-Scramble 2-DXXD conjugates), respectively.
*p<0.05, compared between shLARP7-2 siCtrl and scramble; #p<0.05, compared between shCDK9s and siCtrl.
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