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Each dot represents a single scored cell.
We then scored cell numbers using the In Cell Developer Toolbox (GE Healthcare).
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For these experiments we scored cells from dissociated hindgut/genital ridge prior to cell sorting as a control.
Two days after transfection, we scored cells containing two or more distinct nuclei indicating defective mitosis (Figure 6).
ncell, nROI and nexp, represent the total number of scored cells, total number of detected ROIs and the total number of independent experiments, respectively.
We scored cells with the exception of irradiated cells.
Nuclear abnormalities were scored by immunofluorescence microscopy, numbers of scored cells are indicated within each bar.
The strongly positive descriptor (ezrin overexpression) was assigned to '++' and '+++' scored cells.
The strongly positive descriptor (DEK over expression) was assigned to '++' and '+++' scored cells.
A strongly positive descriptor (LETM1 overexpression) was assigned to "++" and "+++" scored cells.
On the routine tissue sections we calculated an exact ratio from at least 20 scored cells.
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