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Lucida Scorecard test spike (Amersham Life Sciences) was added to 10 μg of total RNA prior to oligo-dT reverse transcription and coupling to Cy3 using an indirect amino amyl procedure.
Briefly, the eluted total RNA was speed vacuumed with a primer annealing mix consisting of a dT/T7 primer, synthetic reference RNA (Lucidea Universal Scorecard reference RNA, Amersham Pharmacia Biotech AB, Uppsala, Sweden) in the case of 10 000 cell samples, and synthetic test RNA (Lucidea Universal Scorecard test RNA, Amersham Pharmacia Biotech AB) plus 40 ng of tRNA in the 1000 and 250 samples.
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Click below to jump to a fact-check: SCORECARD Battle-tested, chief-executive-level experience is Mr. Lhota's ticket to City Hall, which is why it is the focus of his introductory television campaign ad.
Data obtained from the onsite testing sessions will be entered onto a scorecard as the tests are performed and scores made available.
Finally, in the spirit of the old maxim, "What gets measured gets done," we are pilot-testing a scorecard that will allow us and our partners to assess our progress in building sustainable, country-owned health programs.
"It isn't a test or a scorecard of you or your life, so be kind to yourself," she added.
It isn't a test or a scorecard of you or your life, so be kind to yourself ❤️ 11/12.
It will become something of a perfect storm as admission officers seek to bring in classes that test well against scorecard rankings.
The Heritage Action scorecards have become a proxy test of conservatism in recent years, and many Republican members worry which way -- or whether -- Heritage will score a particular vote.
DealBook » Scorecard: Bank Stocks After the Stress Tests | How the stocks of several bank holding companies fared after the Federal Reserve's stress test results were released.
Mr. Norman's scorecard, on a Primary Day that tested not only the mettle of the candidates but the influence of the Democratic leadership, revealed a tally of disappointment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com