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The linear discriminant analysis (LDA) score threshold was set to greater than 3.0.
A score threshold of ≥3 was defined as a 'trigger' as previously described [[16] [18]].
Only bases exceeding the quality score threshold are called; results are summarized in Table S4.
The computer program sought a PWM score threshold to maximize this quantile value.
Additionally, for 13 miRNAs, no targets were predicted at a score threshold of 3.5 (Table 2).
Each point on the ROC curve is a specificity/sensitivity pair corresponding to a score threshold.
We applied a score threshold of 150 to get rid of spurious hits.
At this level of score threshold, the training set gave a FPR of 5%.
We used a stringent SVM score threshold of 0.5 to reduce the number of false positives.
The final score threshold was 2.004.
No score threshold was used for target prediction.
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