Evaluation was done by scoring specimens from 0 to 8 to determine the amount of product present in the specimens, corresponding to each of the time frames.
scored specimens from 71 patients for uPAR-positivity on each of the three cell types at the invasive front and in the tumor core.
Evaluation was done by scoring specimens from 0 to 8 (adapted from the Ashcroft scale for pulmonary fibrosis) to determine the amount of product present in the specimens corresponding to each of the time frames.
We also scored specimens for traits associated with sexual selection including presence and distribution of gular spines (which are only present during the breeding season for species that possess them) and color patterns.
For ER and PR scores, specimens with <1% nuclei staining were considered negative (score 0), and those with weak (1% to 10%, score 1), moderate (10%to33%3%, score 2), and strong (>33%, score 3) staining were classified as hormone-receptor positive, denoted ERP and PRP respectively.
The following classification was used to score surgical specimens for pathological response: CR, no residual invasive carcinoma; PR, residual invasive carcinoma with histological tumour response; and SD, residual invasive carcinoma with no histological tumour response.
Further stratification of PCa samples, based on their Gleason level (GL), outlined that the group of patients with the highest GL, namely GL5, presented with the lowest percentage of score 3 DHRspecimensens (22.2%) (Table 1).
The following classification was used to score histological specimens for pathological response: pCR – no residual invasive carcinoma; pPR – residual invasive carcinoma with histological tumour response; pSD – residual invasive carcinoma with no histological tumour response.
For p16ink4a scoring, 65 specimens were co-scored by two observers blinded to outcome.
For Ki-67 scoring, 108 specimens were co-scored by two observers blinded to outcome (AP and LM).
