The BPMV-GFP construct is an ideal tool to phenotype the 77 RILs since it allowed a visual, rapid and non-destructive scoring of resistance to BPMV.
Cyst formation is typically counted after 4 weeks but because the root tissues used often exhibit significantly reduced vigor 3-4 weeks into such assays, and to expedite and possibly improve resistance scoring, we investigated the scoring of resistance phenotypes at an earlier time point.
Transgenic plants exhibited varied levels (1 2 score on a 0 9 scale) of resistance to BPH, GLH and WBPH on a par with those of BPH-resistant var.
A positive SI score is a measure of sensitivity and a negative SI score is indicative of resistance to paclitaxel treatment.
In addition this study shows that the disease severity index is a better mode of scoring for resistance compared to lesion length.
Using an arbitrary cutoff of 0.25 as the "risk score" of having insulin resistance, nodes ≥25% (proportion of insulin-resistant individuals) predict insulin resistance and nodes <25% predict insulin sensitivity.
A calculation is given as example in Table 1 for tumour probe 2. Please note, that the scores and definition of resistance have been exclusively designed for the present study by L. Edler and W. Rittgen from the Department of Biostatistics, German Cancer Research Center.
Fisher's Least Significant Difference (LSD) test as a comparison of resistance score means revealed no significant differences between the Rar1-silenced plands and other resistant controls but revealed significant differences with the susceptible control, Katahdin.
An average resistance score of 0.1 1.9 was designated as highly resistant (HR), 2.0 3.9 as resistant (R), 4.0 5.9 as moderately resistant (MR), 6.0 7.9 as susceptible (S), and 8.0 9.0 as highly susceptible (HS) (IRRI 2002).
All the improved lines were moderately resistant to ShB compared with the donor parent 'Tetep', which exhibited a higher level of resistance (score 1).
