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These are match score, mismatch score, indel score, probability of match and insertion, minimum score of alignment and the maximum of tandem repeat pattern size.
Next, SAMTools and custom scripts were used to remove (1) incorrectly mapped pairs, (2) pairs in which neither read obtained a Phred score of alignment quality greater than 10, and (3) duplicate fragments (i.e fragments of exactly the same coordinates), which likely represent artifacts of PCR amplification.
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It has been shown, that the numerical score of alignments produced by this heuristic can be far below the optimum [ 5, 13].
An entry M ij ; kl contains the maximal score of alignments of S1[ i.. j] with S2[ k.. l], whereas for the entries D ij ; kl the alignments additionally have to match the base pairs (i, j) and (k, l).
BWA is very fast, maps both short reads and long reads, reads with mismatches and indels and computes the mapping quality score of alignments, and has thus become the read mapping algorithm of choice for many resequencing projects.
The Z scores of Alignment A for comparisons of the Kir family to the other families are also reasonably high (2.2 3.9).
Let γ a, γ b, γ c and γ d be the edge scores of alignment extension, node duplication, edge indel and edge jump, respectively.
For each family of transcription factor matrices in MatBase, we used a one-tailed Wilcoxon test to determine whether or not SCONE scores of alignment positions inside predicted binding sites are higher than SCONE scores outside of sites.
In order to assess the significance of the alignments, we focused on three values of a structure alignment: size of the alignment, size of the double-stranded region of the alignment, and RSmatch score of the alignment.
The gapped extension usually commences from the seed to the partial alignment, since the score of the alignment with the seed is typically much higher than that of the partial alignment.
Second, the generation and scoring of alignments is presented step by step.
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