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These results are consistent with the histological findings, which indicated no change in the severity score for intestinal metaplasia (online appendix table 2).
The slides were reviewed in a blinded fashion by a pathologist (JSD) and were assigned a histological score for intestinal inflammation using a scheme modified from Saverymuttu et al. 24 Data are expressed as means (SEM).
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Overall scores for intestinal symptoms were calculated by frequency and degree.
After incubation at 15°C for 7 days, larval stage F1 progeny were scored for intestinal GFP fluorescence using a Discovery V8 Zeiss fluorescent stereomicroscope.
The overall score of intestinal symptoms was calculated based on a possible score of 36.
Histological scoring of intestinal inflammation for IL-10−/− mice was performed using the scheme previously defined by Madsen et al. [22].
Two independent scoring systems were identified for intestinal ischemia which include the comprehensible parameters of hyperemia and hemorrhage as well as inflammation and epithelial damage and in the case of the higher cited publication by Park et al. several other more sophisticated parameters [ 23, 24].
Overall IBS-QOL score correlated negatively with overall scores of intestinal symptoms and HAMD and HAMA scores (p < 0.001 each).
Next, to determine whether a smaller set of serum factors were predictive biomarkers of colitis, the serum models were refined to a minimal number of input factors that retained predictive and discriminatory power for intestinal lesion scores.
However, for the set of cells labeled in this work, the most salient features of the tissue were modeled directly in the scoring function (e.g. size for intestinal cells).
The groups did not differ in terms of demography, Simplified Acute Physiology Score and risk factors for Candida infection, except for intestinal surgery, exclusive parenteral nutrition and extrarenal epuration.
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