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This was followed by immunofluorescence to score for cells in early, mid, and late S-phase of cell cycle.
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(B ) Distribution of reliability scores for cells pooled from all mice that learned the trace conditioning task.
We overexpressed EGFP-tagged full-length WT or mutant STIL proteins in U2OS cells and scored for cells with more than four centrioles.
In an initial trial, we observed distinct higher clustering scores for cells infected by HCV in comparison to cells infected by the Dengue virus.
Mitochondrial accumulation was scored for cells that had a considerable accumulation of mitochondria around the nucleus, and mitophagy was assessed by the absence of detectable mitochondrial marker staining.
The presence or absence of GATA4 was scored for cells positioned either on the surface or the inside of the ICM.
Since the peak timing reliability scores for cells from trace learners were significantly higher than those for controls, we concluded that only neurons from trace conditioned, learner mice had reliably time-tuned peaks in the ΔF/F trace.
We compared the clustering scores for non-silencing siRNA images of both datasets and observed significantly higher clustering scores for cells with HCV infection (P = 4.8E-4, Wilcoxon test, see Supplementary Fig. S4 for the distribution of all scores for both data sets).
The staining score for tumor cells and normal adjacent cells were recorded separately.
The ratio between the Alamar blue score in IGF1R-overexpressing cells to the score for MCF10A cells grown in Matrigel was calculated.
For each epitope, the staining score for tumor cells and adjacent normal epithelial cells was recorded separately.
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