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She has also given the disease a properly scientific new name: X-associated neuroimmune disease.
Scientific new medicine is one of the most important things" (cited in Yang 2006, 96).
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Could he help me find alternative inspiration, or would his approach prove too pseudo-scientific new age-y for me?
The fermentor scale cultivation was performed at 28°C in a 2.5 L Bioflo III (New Brunswick Scientific, New Brunswick, NJ, USA) fermentor containing 2 L of a sterilized Lindenberg and Holm medium optimized as in [ 21].
Triethlamine (HPLC grade) was obtained from Fisher scientific (New Jersey, USA).
Tigecycline was obtained from Bosche Scientific, New Brunswick, NJ.
Data collection and refinement statistics are summarized in Table 2. Illustrations were made in Pymol (DeLano Scientific, New York NY).
Samples were placed in a New Brunswick Scientific (New Jersey, USA) incubator shaker and hydrolyzed at 50°C and 150 rpm for 72 h.
The amplification products were separated in 1.2% agarose gels containing 0.5 μg/mL of ethidium bromide through electrophoresis in 1X TBE buffer solution at 5 V/cm and visualized under a UVP ultraviolet transilluminator (Spring Scientific, New York, USA).
All experiments used 250 mL flasks placed in a water bath immersion shaker (model G67; New Brunswick Scientific, New Brunswick, NJ), shaken at 110 rpm, and maintained at 37°.
A total of 200 ml of the seed broth was inoculated into a 5-L fermenter (Bioflo 3000 fermenter, New Brunswick Scientific, New Brunswick, NJ, USA) containing 3 L of BSM plus 2 ml/L of PTM1 solution.
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