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The following PCR scheme was used to amplify the genes: one cycle of 95°C for 5 minutes, 10 cycles of 94°C for 20 seconds, 72°C for 30 seconds (with a decrease in temperature of 0.5°C in every cycle) and 72°C for 2 minutes, followed by 35 cycles of 94°C for 20 seconds, 67°C for 30 seconds and 72°C for 2 minutes, and ending with one cycle of 72°C for 7 minutes.
The proposed parametric amplifier scheme can be tuned to amplify a wide range of input frequencies by altering the parametric excitation with no need to physically modify the oscillator.
We also lay out a general scheme to design locus-specific primers to amplify and sequence PPR genes in non-model organisms.
This scheme improves the sensitivity of the receiver in a microwave transport system by using a semiconductor laser to amplify the RF signal.
In the standard spoligotyping scheme, a PCR with primers complementary to the DR-sequence is used to amplify all spacer sequences of a given strain.
Promising to amplify the character of the jumbled complex with a series of surgical interventions, it is the most promising of the six schemes.
We partner with artists to amplify it.
Then they used enzymes to amplify them.
Are you trying to amplify corporate content?
PCR was performed to amplify COI fragment.
4. Forge partnerships to amplify the impact.
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