Sentence examples for sce i from inspiring English sources

Cells have >1 focus when cleaved by I- Sce I near ori, usually 1 focus per cell when cleaved by I- Sce I near ter, far fewer cells with foci when only spontaneous DSBs are present (no I- Sce I cleavage), and <0.03% of cells with foci when GFP alone is produced.

(B – H ) Diagrams of E. coli chromosomes with inducible I- Sce I endonuclease and I-sites engineered (B ) 10 kb, (D ) 55 kb, (F ) 80 kb, and (H ) 2.4 Mb from the tetO array.

The targeting fragment was liberated and linearized in the female germline using FLP and I- Sce I, respectively, and progeny were screened for movement of the mini-white marker to the second chromosome, as well as its resistance to eyFLP (indicating that it is no longer flanked by FRT sites, as in the donor).

DOI: http://dx.doi.org/10.7554/eLife.01222.006 10.7554/eLiFigure2 Figureigure 2—Figure supplement 1. Quantitative real-time PCR shows ∼three-fold more DNA copies near ori than ter in log-phase, regardless of I- Sce I cleavage, implying that some cells have two and some have four ori : ter regions.

In log-phase replicating E. coli, cells have more copies of origin (oriC )-proximal than terminus (ter )-proximal DNA and so will have more DSBs per cell when cleaved by chromosomally encoded I- Sce I (Ponder et al., 2005 ) at a cutsite (red arrow/green flash) near ori than near ter.

(A ) Strategy: we varied the location of I- Sce I cleavage sites (I-sites) in different strains relative to a fixed-position chromosomal TetR-mCherry-bound tetO array, with GamGFP temperature inducibly produced from chromosomal λPR (c I ts857 PR gam-gfp ).

I-Sce I activity led to effective intracellular processing of viral DNA as confirmed by detection of specific cleavage products.

In a proof-of-concept study, we investigated the use of the meganuclease I-Sce I for targeted virus self-disruption to generate high-specific oncolytic viruses.

For this purpose, we provided oncolytic adenoviruses with a molecular circuit that selectively responds to p53 activation by expression of I-Sce I subsequently leading to self-disruption of the viral DNA via heterologous I-Sce I recognition sites within the virus genome.

Besides expressing I-SceI from a plasmid, we also show that adding I-SceI enzyme during transformation was successful to generate DSBs.

We previously showed that creation of a double strand DNA break (DSB) by expressing I-SceI in an engineered Trichoderma reesei (Hypocrea jecorina) strain containing a I-SceI recognition site improved transformation and homologous integration efficiencies.