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The scavenging effect was obtained from the following relation: (7) Scavenging effect = (A 0 − A 1 ) × 100 A 0, where A0 was the absorbance of the control reaction and A1 was the absorbance of the sample of the tested extracts.
In addition, the antioxidant activity of the compounds was also investigated through scavenging effect on DPPH radicals.
Therefore, the HA-enhanced effect should be ascribed to the hole scavenging effect.
Table 2 shows the IC50 of (OH) scavenging effect by various fractions along with ascorbic acid.
The scavenging effect on DPPH radical was varied significantly by different fractions (Table 4).
Fig. 4 Dose-dependence of the superoxide anion scavenging effect of the MeOH extracts and fractions from Geum urbanum.
Radical scavenging effect of the aqueous extract and SNPs/extract mixture, were evaluated by DPPH scavenging assay.
The authors noted scavenging effect of excess H2O2 due to its reaction with HO·, inhibiting the oxidation process.
The ability to scavenge DPPH radicals was calculated using the equation: scavenging effect = [1 – (absorbance of sample at 517 nm/absorbance of control at 517 nm)] × 100%.
T100 and T75 showed the highest antioxidant activity, scavenging effect on free radicals and FRAP compared to control (CF) and other treatments (P≤ 0.05).
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It was found that the higher the concentration of A. camphorata extracts, the higher the DPPH radical-scavenging effect.
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