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c, Left, scattering profile of SdeA213 907 with calculated scattering curve from crystal structure.
In the heating process, the Hv light scattering intensity started to decrease at low temperature far below the melting temperature while the ordering of the crystallites, estimated by the broadening of the light scattering profile, did not change up to the vicinity of the melting temperature.
The parameters obtained from the analysis of the SANS scattering profile at pH 4 was also listed in Table 2.
An exponential-decay Mie scattering profile with decreasing photon energy is clearly observable.
The normalized background scattering profile of the buffer and polycarbonate cell was subtracted from the normalized sample scattering profiles to obtain the protein scattering curve.
According to the mean-field theory of polymers in a good solvent [ 48, 49], scattering profile can be described in terms of the mesh size formed by cross-links.
Information about the structure of scattering objects is usually obtained by comparing a scattering profile calculated from a presumed geometrical shape with the obtained data, and the process is iterated until an acceptable fit is produced.
Addition of 1.7 M NaCl did not alter the main feature of the picture, that is, the two types of scattering objects were still required to fit the measured scattering profile.
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In the presence of the radical scavenging agents Tris-HCl and ascorbic acid (Fig. 1A), the nanocrystal auto-scattering profile was stable for more than 200 seconds on the high-flux BESSRC-CAT 12ID-C beamline.
Second, the two single-labeled DNA scattering intensity profiles (IA and IB) and the double-labeled DNA scattering intensity profile (IAB) were buffer-subtracted and scaled so as to superimpose onto the tail of the gold auto-scattering profile at S≥0.04 Å−1.
We determined the size distribution of our nanocrystal preparation by decomposing the auto-scattering profile into a linear combination of basis profiles corresponding to hard sphere scatterers of varying diameter.
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