However, these studies, based on light scattering, implied that polymerization is not inhibited by SlmA, which appeared to be contrary to the mechanism of NO (Bernhardt and de Boer, 2005).
In light scattering assays using standard polymerization buffer of pH 6.5, B. subtilis FtsZ gave a classic response upon the addition of GTP, with a strong increase in light scatter signal that decreased over time (Supplementary Figure S3) (Mukherjee and Lutkenhaus, 1999).
In addition, it turned out that in a plot of Tg against dyad or (syndiotactic) triad fraction, a linear correlation can be expected only for steric copolymers containing negligible amounts of the heterotactic triad and, in general, the data are scattered due to different polymerization kinetics.
Bond and co-workers used X-ray crystallography and small angle X-ray scattering (SAXS) to study the polymerization of DBHS family of splicing factors, localized to and enriched in paraspeckles [ 19, 70].
(A ) Right-angle light scattering traces of D190A-PhuZ polymerization at 1.5 (purple), 2 (green), 2.5 (red), and 3 (blue) μM upon addition of 1 mM GTP. (B ) Right-angle light scattering traces of PhuZ (red) or D190A-PhuZ (green) polymerized in 100 μM GTP. 3 mM GDP was added after polymerization reached steady state (arrow + GDP), and subsequent depolymerization was monitored.
The classic light scattering studies of Hantgan and Hermans showed polymerization occurs in two steps: end-to-end polymerization of monomers into protofibrils and lateral association of protofibrils into fibers.
GPC, 1H NMR, TEM and laser light scattering (LLS) were used to track the polymerization.
However, we find that the capsid protein readily forms pentamers of dimers in solution, based on polymerization kinetics observed by light scattering.
The effects of copolymer composition and polymerization temperature on the light-scattering properties were investigated.
