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Increased MUC13 expression has been found to induce morphological changes, including scattering of cells through interference with the function of cell adhesion molecules [14]; thus, an increased expression along with differentiation may indicate enhanced cell-cell adhesion.
Neither does this mutation result in the random scattering of cells that is observed for the dentate granule cells.
Therefore, standard FC does not faithfully detect DNA content heterogeneity or scattering of cells in the histogram.
HGF binds c-Met, a tyrosine kinase receptor as exclusive ligand induces the activation of oncogenic pathways, angiogenesis and scattering of cells, leading to metastasis [ 32].
We next investigated whether the reduced scattering of cells expressing the 4x595R ubiquitylation defective TIAM1 mutant would lead to impaired cell invasion.
Although not very strong, Ep-CAM mediated adhesions were able to suppress the scattering of cells embedded into matrigel (Litvinov, 1995).
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We found that maintaining Hnf6 expression in islets resulted in a host of morphological and functional deficiencies, including a scattering of cell types within the islets, abnormal islet size, decreased insulin secretion, and a block to β cell maturation (Figure 1; [22], [22]).
The decrease in the forward light scatter of cells treated with H35N in presence of zVADfmk indicated that the cell death resembled apoptosis (Fig. 6B-D).
However, in our flow cytometry studies curcumin-treatment did not change the forward scatter of cells, indicating that cell size was unaltered (data not shown).
We therefore analysed the same samples shown in Figure 5, and as Figure 6 shows, we detected in one wild-type line, 17AS, a significant increase (P=0.0001) in the forward and side scatter of cells in response to exposure to 6 Gy of ionising radiation in cells pre-treated with Nutlin-3 (this was not significant in the other wild-type cell line, 17A; P=0.08).
Our group previously showed that ezrin acts co-operatively with the non-receptor tyrosine kinase, Src, in deregulation of cell-cell contacts and scattering of epithelial cells.
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