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Analysis shows that the measured elastic light scattering distributions reveal changes in the RBC's orientation and shape.
With the framework above, we simulated multiple-illuminations of 2D absorption and scattering distributions 2 cm × 2 cm in a grid of 30 × 30 pixels of size Δ x = Δ y = 667 μ m.
Thus, the results show that the linear reconstruction method with a reference measurement correction can reconstruct the absorption and scattering distributions well if the background optical properties are known.
The angular scattering distributions that are observed for the various inelastic scattering channels qualitatively follow the trend that is typically observed for inelastic collisions between atoms and diatomic molecules.
To quantitatively compare experimental and simulated scattering images in Fig. 4, our first task is to extract the angular scattering distributions from the images by analyzing the scattering intensity in a small annulus around the rims of the images.
Figure 7 Angular scattering distributions.
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Here, we will not present the results of the uniform (although limited) azimuth/elevation scattering distribution function presented in [12].
Figure 4 shows the daily light scattering distribution determined with a nephelometer at 550 nm.
Cox et al. [ 12] recovered both absorption and scattering distribution with multiple optical wavelengths when known wavelength dependence of the optical scattering exists.
Another laser (He-Ne) was used to illuminate the cell and elastic light scattering distribution from the single cell was measured with a goniometer and a photomultiplier tube.
Figure 3 (b) shows a trapped spherical RBC and 3 (c) shows the light scattering distribution under He-Ne laser illumination.
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