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The cell sizes were inversely proportional to the amount of light scattered forward and at right angles.
The ground further in front of the aircraft also caused returns, but these were increasingly distant (see slant range) and only some of the signal was reflected back at the aircraft while an increasing portion was scattered forward and away.
In flow cytometry, dead cells can be identified on the basis of changes in scattering by plotting the intensity of light scattered forward and at 90° [ 28], and the formation of apoptotic bodies can be characterized by a decrease in both forward and side scattering [ 40].
For penetration of white Caucasian skin by UV rays total transmission coefficients was used (directly transmitted light plus that scattered forward).
Similar(56)
Signals were amplified with the logarithmic mode for side scattering, forward scattering, and FITC fluorescence.
Side scatter, forward scatter, and pulse-width gates excluded debris and aggregates.
Samples were analysed on a CyAn ADP high-performance flow cytometer (Becton Dickinson, Oxford, UK); cells were gated according to size scattering, forward scattering and pulse width so only single cells were analysed.
Inflammatory cell populations (monocytes, neutrophils, eosinophils, and lymphocytes) in blood were identified and gated based on light scatter properties (forward scatter and side scatter).
Side scatter versus forward scatter dot plots were used to locate and gate nuclear populations by particle size.
Cells were gated in a SSC (side scatter)/FSC (forward scatter) plot with the scatter gate for monocytes partially extending into lymphocytes [ 25].
The dot plots in Fig. 3A show differences in side scatter and forward scatter profiles between unprimed and primed cells cultured with infected PL's for 72hrs.
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