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Fifty pelvic CT scans were selected from a large database.
31 F-FDG-PET/CT scans were selected from 2 databases.
31 FDG-PET/CT scans were selected from the databases of the Department of Nuclear Medicine and PET Research of the VU University Medical Center (VUMC) and the Department of Nuclear Medicine and Molecular Imaging of the University Medical Center Groningen (UMCG).
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From these video recordings, representative horizontal and longitudinal scans were selected for assessment.
The four most intense precursor ions from each full scan were selected for MS/MS.
The 3 most abundant ions from a full scan were selected for fragmentation via collision-induced dissociation.
For each cycle, the five most abundant ions from each MS scan were selected for MS/MS analysis using 20% normalized collision energy.
For each MS cycle, the 10 highest intense precursor ions from a survey scan were selected for MS/MS.
Up to 20 and 10 most intense precursor ions from the full scan were selected for fragmentation by CID and HCD, respectively.
MS/MS spectra were acquired in a data-dependent mode in which up to four precursor ions above an intensity threshold of seven counts/second (cps) from each survey "scan" were selected for MS/MS analysis.
For each duty cycle, twenty most intense precursor ions from a survey scan were selected for MS/MS and detected at a mass resolution of 15,000 at m/z of 400, also in an Orbitrap analyzer.
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