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Separate random sample of 10 MRI scans were selected for tissue segmentations of cerebral GM, cerebral WM, and CSF (ventricular and subarachnoid).
From these video recordings, representative horizontal and longitudinal scans were selected for assessment.
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The four most intense precursor ions from each full scan were selected for MS/MS.
The 3 most abundant ions from a full scan were selected for fragmentation via collision-induced dissociation.
For each cycle, the five most abundant ions from each MS scan were selected for MS/MS analysis using 20% normalized collision energy.
The mass spectrometer was operated in a data-dependent mode where the three most intense ions detected in each MS scan were selected for tandem MS (MS/MS) in the linear ion trap.
The top five ions in each MS1 scan were selected for MS/MS fragmentation.
The five most abundant peptides in each MS scan were selected for fragmentation.
For each MS cycle, the 10 highest intense precursor ions from a survey scan were selected for MS/MS.
Low frequency/rare variants contributing to prioritised association signals in the primary scan were selected for follow-up.
Up to 20 and 10 most intense precursor ions from the full scan were selected for fragmentation by CID and HCD, respectively.
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