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Densitometry scans were generated from the Western blots and the specific bands are shown in Figure 3b.
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Slides were scanned for Cy5 and Cy3 fluorescence using an Affymetrix 428 array scanner, and.tif images were generated from scans for both channels.
Data files were generated from scanned array images in the Affymetrix Microarray Suite Version 4.0 program.
Present/Absent/Marginal calls were generated from scanned arrays using Affymetrix® GCOS 1.4 software.
CEL files were generated from scanned images using GeneChip™ Operating Software (GCOS, Affymetrix, version 1.3.0.037).
A detailed description of sample processing is available in Additional file 1. CEL files were generated from scanned images (DAT files) using GCOS 1.4 software (Affymetrix).
X-ray computed tomographic (CT) scans of the braincase (Figures 12 16) were generated from data gathered at the North Carolina State University College of Veterinary Medicine and edited in OsiriX [57].
Firstly, 3D digital tibial models were generated from CT scans.
The initial 3D solid mandible (including the separation between cortical and cancellous bone) and parts of the temporal bone models were generated from CT scan data, and the solid models of the glenoid fossa, condyle, and articular disk were built from the MRI scan data.
Three-dimensional bone models of the affected and contralateral healthy lower limb were generated from computed tomography scans.
The time-activity curves (TACs) were generated from dynamic PET scans.
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