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For the estimation of HOMO and LUMO energy levels, cyclic voltammetry (CV) scans were conducted using a Gamry 600 reference potentiostat (Gamry Instruments, Inc., Warminster, USA) equipped with virtual front panel software.
All AFM scans were conducted using an Innova scanning probe microscope (Veeco, U.S.A .. Scans were performed in tapping mode at ambient temperature and pressure, using silicon cantilevers (MPP-31120-10, Veeco, U.S.A).
After an intravenous injection of 11C-PBB3 (injectedosese, 399 ± 45 MBq; specific activity, 88 ± 32 GBq/μmol), 70-min dynamic PET scans were conducted using an ECAT Exact HR+ system (developed by CTI, Knoxville, TN, USA, and distributed by Siemens Healthcare, Erlangen, Germany).
All BMD scans were conducted using standardized procedures following the manufacturer's recommended protocols by well-trained examiners.
All PET scans were conducted using an ECAT HR+ 962 scanner (CTI/Siemens, Knoxville, TN, USA), with an axial field of view of 15.5 cm.
For functional localization of V5, four functional scans were conducted using a T2*-weighted gradient echo, EPI sequence (TR = 2 s, TE = 30 ms, and flip angle = 65°) to acquire 160 volumes constructed from 39 axial slices covering the whole brain at voxel resolution of 3 × 3 × 3 mm.
Similar(54)
Hybridisation, washing, staining and scanning were conducted using the same instruments for all samples.
Vehicle or acetaminophen at a dose of 100 or 300 mg/kg was intraperitoneally administered, and PET scanning was conducted using 18F-BCPP-BF 2 or 24 h after the administration of acetaminophen.
Scanning was conducted using a GeneChip Scanner 3000.
A genome scan was conducted using the same methods as above.
The scan was conducted using HMMSearch from profile HMM package HMMER, obtained from Washington University [ 38].
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