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Use of PET scans was first incorporated into NCCN guidelines in 2003, when they were described as an optional imaging procedure for stage IV metastatic or recurring breast cancer; however, the guidelines recommended that PET scans not replace more established imaging methods.

Low dose CT scans were first rebinned (with in-house developed software) using trilinear interpolation with a 4 × 4 × 4 mm3 voxel size in order to match matrix and voxel size of the PET images (Fig. 1a).

The remaining 230 functional scans were first corrected for within-scan acquisition time differences between slices and realigned to the first volume to correct for inter-scan movements.

For each RIS and RRMS subject, MR scans were first visually assessed by a single observer (FR) who was unaware of subject identity.

Anatomical scans were first acquired and if excessive motion was identified, a second acquisition was attempted.

The 'tag', 'control', and M0 scans were first motion-corrected using MCFLIRT (Jenkinson et al., 2002).

In addition, in all trauma centers, scans are first interpreted by radiologists and further reviewed by clinicians in charge of the patients (emergency physicians or surgeons or both).

To create the customized template, all scans were first segmented into gray, white and cerebrospinal fluid (CSF) compartments in their native space using the standard SPM2 templateate (MNI305 [ 68]).

T1-weighted volumetric MRI scans were first preprocessed according to a standardized protocol for alignment, removal of extracranial material, and segmentation of brain into gray and white parenchyma and cerebrospinal fluid.

In a second animal, a baseline [11C]PBR28 scan was first acquired.

A 24-min baseline Tc-99 m MAG3 scan was first obtained.