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During scans, animals received 0.2 mg/kg apomorphine, i.v. In a subset of experiments, animals were pretreated with 2 mg/kg of the D2R antagonist, eticlopride.Following apomorphine administration, BOLD signal decreases were observed in caudate/putamen of WT and KO animals.
Between scans, animals underwent one week of recovery.
For in vivo SPECT/CT scans, animals were anesthetized with isoflurane (1.5 4 %) in oxygen.
Immediately after MRI scans, animals remained under anesthesia in the same position and the cradle was transferred to the PET scanner.
Prior to DCE-MRI scans, animals were anesthetized with isoflurane (4%), intubated, and maintained under anesthesia (2 2.5% isoflurane, 30% oxygen, 67.5 68% air) by mechanical ventilation through a rodent ventilator for the entire duration of the scan (approximately 2 h).
Immediately after the ERG scans, animals were sacrificed with pentobarbital sodium (> 60 mg/kg, intraperitoneal) and eyes were enucleated.
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Harris offers a variety of interesting evidence for these systems: brain scans, animal studies and neurological diseases that knock out one system but not the other.
Of the animals that had two scans, one underwent two post-tariquidar scans (animal 4, Table 1).
Table 1 Overview of [ 11 C]phenytoin scans Animal Scan 1 Scan 2 Scan 3 1 Baseline - - 2 Baseline - - 3 Baseline Tariquidar 15 mg·kg−1 - 4 Tariquidar 15 mg·kg−1 Tariquidar 15 mg·kg−1 - 5 Baseline Probenecid 150 mg·kg−1 Tariquidar 15 mg·kg−1 6 Baseline Probenecid 150 mg·kg−1 Tariquidar 15 mg·kg−15
Computed tomography (CT) scan: animals underwent two CT scans (Skyscan 1176, Bruker, Brussels, Belgium).
Throughout the MR scan, animals were under continuous 1.0% to 1.8% isoflurane anesthesia with 2 L/min oxygen flow.
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