The sweep time was set at 84 s and the number of scans was fixed at 10.
Since the direction of the A-scans is fixed along the z-direction the B-scan planes are always vertical.
While the resolution of the CT scan depends on the CT machine type and settings, the resolution for a given set of scans is fixed.
And the scan sizes were fixed at 39.9 × 39.9 μm and 10.0 × 10.0 μm, and the height of the surface relief was recorded at a resolution of 256 × 256 pixels.
For scanning microscopy, leaves were fixed with 3% glutaldeihyde in 0.1 M sodium cacodylate buffer at pH 7.2 for 15 h at 4°C and postfixed in 1% osmium tetroxide for 1 h.
For scanning electron microscopy, gynoecia were fixed in Karnovsky's fixative (4 h, 4 °C), dehydrated using a cold acetone series (from 10to100%0 %), dried to the critical point E-30000, Quorum Technologies, UK), sputter-coated with gold palladium (JFC-1600 Autofine Coater, Jeol, Japan) and observed under a scanning electron microscope (JSM-6610LV-Jeol, Japan).
For scanning electron microscopy cells were fixed in glutaraldehyde, dehydrated in a graded series of ethanol and critical point dried.
For scanning electron microscopy cells were fixed in situ with 1.6% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.5) at room temperature.
For scanning electron microscopy, samples were fixed overnight at 4°C in 2.5% glutaraldehyde 0.1 M cacodylate buffer (pH 7.2) and postfixed in 2% OsO4 in the same buffer.
For scanning electron microscopy, samples were fixed for 2 h with Karnovsky 30, and postfixation was performed with 1% osmium tetroxide (buffered at pH 6.5 with 0.1 M sodium cacodylate) for further 2 h.
